Description:Rabbit polyclonal antibody to UBE2G2Immunogen:Recombinant full length protein of human UBE2G2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:UBE2G2Alternative Names:Ubiquitin-conjugating enzyme E2 G2; Ubiquitin carrier protein G2; Ubiquitin-protein ligase G2
Entrez Gene (Human):
7327;
Entrez Gene (Mouse):
22213;
SwissProt (Human):
P60604;
SwissProt (Mouse):
P60605;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of UBE2G2 expression in MCF7 (A), HT1080 (B), mouse heart (C), rat testis (D) whole cell lysates. (Predicted band size: 15; 18 kD; Observed band size: 18 kD)
-
Immunofluorescent analysis of UBE2G2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).