Description：Rabbit polyclonal antibody to EIF3BImmunogen：Recombinant full length protein of human EIF3BPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：EIF3BAlternative Names：EIF3S9; Eukaryotic translation initiation factor 3 subunit B; eIF3b; Eukaryotic translation initiation factor 3 subunit 9; Prt1 homolog; hPrt1; eIF-3-eta; eIF3 p110; eIF3 p116 Entrez Gene (Human)： 8662; Entrez Gene (Mouse)： 27979; Entrez Gene (Rat)： 288516; SwissProt (Human)： P55884; SwissProt (Mouse)： Q8JZQ9; SwissProt (Rat)： Q4G061; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EIF3B expression in Hela (A), Jurkat (B), NIH3T3 (C), rat thymus (D) whole cell lysates. (Predicted band size: 92; 99 kD; Observed band size: 125 kD)
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  Immunofluorescent analysis of EIF3B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).