Description：Rabbit polyclonal antibody to SFPQImmunogen：Recombinant full length protein of human SFPQPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：SFPQAlternative Names：PSF; Splicing factor, proline- and glutamine-rich; 100 kDa DNA-pairing protein; hPOMp100; DNA-binding p52/p100 complex, 100 kDa subunit; Polypyrimidine tract-binding protein-associated-splicing factor; PSF; PTB-associated-splicing factor Entrez Gene (Human)： 6421; Entrez Gene (Mouse)： 71514; SwissProt (Human)： P23246; SwissProt (Mouse)： Q8VIJ6; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SFPQ expression in Jurkat (A), HepG2 (B), mouse liver (C), rat brain (D) whole cell lysates. (Predicted band size: 72; 76 kD; Observed band size: 115 kD)
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  Immunohistochemical analysis of SFPQ staining in human esophageal formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of SFPQ staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).