Description：Rabbit polyclonal antibody to NMDAR2AImmunogen：Recombinant full length protein of human NMDAR2APurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：GRIN2AAlternative Names：NMDAR2A; Glutamate receptor ionotropic, NMDA 2A; GluN2A; Glutamate [NMDA] receptor subunit epsilon-1; N-Methyl-D-aspartate receptor subtype 2A; NMDAR2A; NR2A; hNR2A Entrez Gene (Human)： 2903; Entrez Gene (Mouse)： 14811; Entrez Gene (Rat)： 24409; SwissProt (Human)： Q12879; SwissProt (Mouse)： P35436; SwissProt (Rat)： Q00959; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NMDAR2A expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 144; 165 kD; Observed band size: 180 kD)
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  Immunofluorescent analysis of NMDAR2A staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).