Description：Rabbit polyclonal antibody to GTF2IImmunogen：KLH-conjugated synthetic peptide of human GTF2IPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：GTF2IAlternative Names：BAP135; WBSCR6; General transcription factor II-I; GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region 6 protein Entrez Gene (Human)： 2969; Entrez Gene (Mouse)： 14886; Entrez Gene (Rat)： 353256; SwissProt (Human)： P78347; SwissProt (Mouse)： Q9ESZ8; SwissProt (Rat)： Q5U2Y1; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GTF2I expression in HEK293T (A) whole cell lysates. (Predicted band size: 30; 107; 110; 112 kD; Observed band size: 135 kD)
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  Immunofluorescent analysis of GTF2I staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).