Description：Rabbit polyclonal antibody to NEDD4Immunogen：Recombinant full length protein of human NEDD4Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol：NEDD4Alternative Names：KIAA0093; NEDD4-1; E3 ubiquitin-protein ligase NEDD4; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4 Entrez Gene (Human)： 4734; Entrez Gene (Mouse)： 17999; Entrez Gene (Rat)： 25489; SwissProt (Human)： P46934; SwissProt (Mouse)： P46935; SwissProt (Rat)： Q62940; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NEDD4 expression in Hela (A), Jurkat (B), A549 (C), SKOV3 (D) whole cell lysates. (Predicted band size: 104; 140; 147; 149 kD; Observed band size: 120 kD)
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  Immunohistochemical analysis of NEDD4 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.