Description：Rabbit polyclonal antibody to TRPV5Immunogen：Recombinant full length protein of human TRPV5Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)Gene Symbol：TRPV5Alternative Names：ECAC1; Transient receptor potential cation channel subfamily V member 5; TrpV5; Calcium transport protein 2; CaT2; Epithelial calcium channel 1; ECaC; ECaC1; Osm-9-like TRP channel 3; OTRPC3 Entrez Gene (Human)： 56302; Entrez Gene (Mouse)： 194352; Entrez Gene (Rat)： 116469; SwissProt (Human)： Q9NQA5; SwissProt (Mouse)： P69744; SwissProt (Rat)： Q9JIP0; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRPV5 expression in HepG2 (A), HT29 (B), mouse kidney (C) whole cell lysates. (Predicted band size: 43; 82 kD; Observed band size: 83 kD)
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  Immunofluorescent analysis of TRPV5 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.