Description:Rabbit polyclonal antibody to TIA1Immunogen:Recombinant full length protein of human TIA1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:TIA1Alternative Names:Nucleolysin TIA-1 isoform p40; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; p40-TIA-1
Entrez Gene (Human):
7072;
Entrez Gene (Mouse):
21841;
SwissProt (Human):
P31483;
SwissProt (Mouse):
P52912;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TIA1 expression in SKOV3 (A), Hela (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 24; 41; 42 kD; Observed band size: 44 kD)
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Immunohistochemical analysis of TIA1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.