Description:Rabbit polyclonal antibody to SIRT4Immunogen:KLH-conjugated synthetic peptide of human SIRT4Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:SIRT4Alternative Names:SIR2L4; NAD-dependent protein deacetylase sirtuin-4; NAD-dependent ADP-ribosyltransferase sirtuin-4; Regulatory protein SIR2 homolog 4; SIR2-like protein 4
Entrez Gene (Human):
23409;
Entrez Gene (Mouse):
75387;
SwissProt (Human):
Q9Y6E7;
SwissProt (Mouse):
Q8R216;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SIRT4 expression in A549 (A), NIH3T3 (B), mouse testis (C) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 35 kD)
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Immunohistochemical analysis of SIRT4 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.