Description:Rabbit polyclonal antibody to RPLP0Immunogen:Recombinant full length protein of human RPLP0Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:RPLP0Alternative Names:60S acidic ribosomal protein P0; 60S ribosomal protein L10E
Entrez Gene (Human):
6175;
Entrez Gene (Mouse):
11837;
Entrez Gene (Rat):
64205;
SwissProt (Human):
P05388;
SwissProt (Mouse):
P14869;
SwissProt (Rat):
P19945;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RPLP0 expression in A549 (A), K562 (B), Jurkat (C), mouse liver (D), mouse testis (E) whole cell lysates. (Predicted band size: 27; 34 kD; Observed band size: 37 kD)
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Immunofluorescent analysis of RPLP0 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.