Description:Rabbit polyclonal antibody to RNF5Immunogen:Recombinant full length protein of human RNF5Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:RNF5Alternative Names:G16; NG2; RMA1; E3 ubiquitin-protein ligase RNF5; Protein G16; RING finger protein 5; Ram1 homolog; HsRma1
Entrez Gene (Human):
6048;
Entrez Gene (Mouse):
54197;
Entrez Gene (Rat):
407784;
SwissProt (Human):
Q99942;
SwissProt (Mouse):
O35445;
SwissProt (Rat):
Q5M807;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RNF5 expression in MCF7 (A), Jurkat (B), mouse liver (C), mouse kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 19 kD; Observed band size: 21 kD)
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Immunohistochemical analysis of RNF5 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.