Description:Rabbit polyclonal antibody to Peroxiredoxin 6Immunogen:Recombinant full length protein of human Peroxiredoxin 6Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol:PRDX6Alternative Names:AOP2; KIAA0106; Peroxiredoxin-6; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; Liver 2D page spot 40; Non-selenium glutathione peroxidase; NSGPx; Red blood cells page spot 12
Entrez Gene (Human):
9588;
Entrez Gene (Mouse):
102642619;
11758;
SwissProt (Human):
P30041;
SwissProt (Mouse):
O08709;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Peroxiredoxin 6 expression in PC3 (A), K562 (B), mouse liver (C), mouse heart (D) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 27 kD)
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Immunofluorescent analysis of Peroxiredoxin 6 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.