Description：Rabbit polyclonal antibody to PPP2R1AImmunogen：Recombinant full length protein of human PPP2R1APurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：PPP2R1AAlternative Names：Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha Entrez Gene (Human)： 5518; Entrez Gene (Mouse)： 51792; SwissProt (Human)： P30153; SwissProt (Mouse)： Q76MZ3; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PPP2R1A expression in SKOV3 (A), HepG2 (B), mouse brain (C), mouse kidney (D) whole cell lysates. (Predicted band size: 65 kD; Observed band size: 65 kD)
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  Immunofluorescent analysis of PPP2R1A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).