Description：Rabbit polyclonal antibody to DAX1Immunogen：Recombinant full length protein of human DAX1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)Gene Symbol：NR0B1Alternative Names：AHC; DAX1; Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1 Entrez Gene (Human)： 190; Entrez Gene (Mouse)： 11614; Entrez Gene (Rat)： 58850; SwissProt (Human)： P51843; SwissProt (Mouse)： Q61066; SwissProt (Rat)： P70503; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DAX1 expression in Jurkat (A), MCF7 (B), HepG2 (C), mouse spleen (D) whole cell lysates. (Predicted band size: 43; 51 kD; Observed band size: 50 kD)
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  Immunofluorescent analysis of DAX1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.