Description:Rabbit polyclonal antibody to NDUFB5Immunogen:Recombinant full length protein of human NDUFB5Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/100)Gene Symbol:NDUFB5Alternative Names:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5 mitochondrial; Complex I-SGDH; CI-SGDH; NADH-ubiquinone oxidoreductase SGDH subunit
Entrez Gene (Human):
4711;
Entrez Gene (Mouse):
66046;
SwissProt (Human):
O43674;
SwissProt (Mouse):
Q9CQH3;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NDUFB5 expression in HepG2 (A), SW480 (B), mouse liver (C), mouse brain (D), rat kidney (E) whole cell lysates. (Predicted band size: 15; 21 kD; Observed band size: 22 kD)
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Immunohistochemical analysis of NDUFB5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.