Description:Rabbit polyclonal antibody to NRAGEImmunogen:Recombinant full length protein of human NRAGEPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:MAGED1Alternative Names:NRAGE; Melanoma-associated antigen D1; MAGE tumor antigen CCF; MAGE-D1 antigen; Neurotrophin receptor-interacting MAGE homolog
Entrez Gene (Human):
9500;
Entrez Gene (Mouse):
94275;
Entrez Gene (Rat):
84469;
SwissProt (Human):
Q9Y5V3;
SwissProt (Mouse):
Q9QYH6;
SwissProt (Rat):
Q9ES73;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NRAGE expression in A549 (A) whole cell lysates. (Predicted band size: 86; 91 kD; Observed band size: 86 kD)
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Immunohistochemical analysis of NRAGE staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.