Description:Rabbit polyclonal antibody to LITAFImmunogen:Recombinant full length protein of human LITAFPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:LITAFAlternative Names:PIG7; SIMPLE; Lipopolysaccharide-induced tumor necrosis factor-alpha factor; LPS-induced TNF-alpha factor; Small integral membrane protein of lysosome/late endosome; p53-induced gene 7 protein
Entrez Gene (Human):
9516;
Entrez Gene (Mouse):
56722;
Entrez Gene (Rat):
65161;
SwissProt (Human):
Q99732;
SwissProt (Mouse):
Q9JLJ0;
SwissProt (Rat):
P0C0T0;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LITAF expression in K562 (A), Hela (B), HepG2 (C) whole cell lysates. (Predicted band size: 15; 17; 23 kD; Observed band size: 23 kD)
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Immunofluorescent analysis of LITAF staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.