Description：Rabbit polyclonal antibody to Importin alpha 1Immunogen：Recombinant full length protein of human Importin alpha 1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：KPNA1Alternative Names：RCH2; Importin subunit alpha-5; Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1; RAG cohort protein 2; SRP1-beta Entrez Gene (Human)： 3836; Entrez Gene (Mouse)： 16646; SwissProt (Human)： P52294; SwissProt (Mouse)： Q60960; SwissProt (Rat)： P83953; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Importin alpha 1 expression in HepG2 (A), Hela (B), A549 (C), mouse brain (D), mouse heart (E) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 60 kD)
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  Immunohistochemical analysis of Importin alpha 1 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Importin alpha 1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.