Description:Rabbit polyclonal antibody to Kinesin 2Immunogen:Recombinant full length protein of human Kinesin 2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol:KLC1Alternative Names:KLC; KNS2; Kinesin light chain 1; KLC 1
Entrez Gene (Human):
3831;
Entrez Gene (Rat):
171041;
SwissProt (Human):
Q07866;
SwissProt (Mouse):
O88447;
SwissProt (Rat):
P37285;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Kinesin 2 expression in SW480 (A), MCF7 (B), mouse brain (C) whole cell lysates. (Predicted band size: 62-72 kD; Observed band size: 65 kD)
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Immunofluorescent analysis of Kinesin 2 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.