Description：Rabbit polyclonal antibody to IL-18Immunogen：Recombinant full length protein of human IL-18Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/50 - 1/100)Gene Symbol：IL18Alternative Names：IGIF; IL1F4; Interleukin-18; IL-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma Entrez Gene (Human)： 3606; Entrez Gene (Mouse)： 16173; Entrez Gene (Rat)： 29197; SwissProt (Human)： Q14116; SwissProt (Mouse)： P70380; SwissProt (Rat)： P97636; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IL-18 expression in Hela (A) whole cell lysates. (Predicted band size: 21; 22 kD; Observed band size: 22 kD)
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  Immunofluorescent analysis of IL-18 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).