Description：Rabbit polyclonal antibody to HSD17B2Immunogen：Recombinant full length protein of human HSD17B2Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：HSD17B2Alternative Names：EDH17B2; Estradiol 17-beta-dehydrogenase 2; 17-beta-hydroxysteroid dehydrogenase type 2; 17-beta-HSD 2; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; Microsomal 17-beta-hydroxysteroid dehydrogenase; Testosterone 17-beta-dehydrogenase Entrez Gene (Human)： 3294; Entrez Gene (Mouse)： 15486; Entrez Gene (Rat)： 79243; SwissProt (Human)： P37059; SwissProt (Mouse)： P51658; SwissProt (Rat)： Q62730; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSD17B2 expression in SW480 (A), mouse liver (B), mouse kidney (C) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 43 kD)
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  Immunohistochemical analysis of HSD17B2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of HSD17B2 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.