Description：Rabbit polyclonal antibody to HeparanaseImmunogen：Recombinant full length protein of human HeparanasePurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：HPSEAlternative Names：HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Heparanase; Endo-glucoronidase; Heparanase-1; Hpa1 Entrez Gene (Human)： 10855; Entrez Gene (Mouse)： 15442; Entrez Gene (Rat)： 64537; SwissProt (Human)： Q9Y251; SwissProt (Mouse)： Q6YGZ1; SwissProt (Rat)： Q71RP1; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Heparanase expression in Hela (A), MCF7 (B), mouse liver (C), mouse lung (D) whole cell lysates. (Predicted band size: 42; 53; 54; 61 kD; Observed band size: 61; 50 kD)
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  Immunofluorescent analysis of Heparanase staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).