Description:Rabbit polyclonal antibody to HAPLN1Immunogen:Recombinant full length protein of human HAPLN1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:HAPLN1Alternative Names:CRTL1; Hyaluronan and proteoglycan link protein 1; Cartilage-linking protein 1; Cartilage-link protein; Proteoglycan link protein
Entrez Gene (Human):
1404;
Entrez Gene (Mouse):
12950;
Entrez Gene (Rat):
29331;
SwissProt (Human):
P10915;
SwissProt (Mouse):
Q9QUP5;
SwissProt (Rat):
P03994;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HAPLN1 expression in BT474 (A), MCF7 (B), Hela (C), HepG2 (D) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 50 kD)
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Immunohistochemical analysis of HAPLN1 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.