Datasheet
MSDSDescription：Rabbit polyclonal antibody to EIF6Immunogen：Recombinant full length protein of human EIF6Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：EIF6Alternative Names：EIF3A; ITGB4BP; Eukaryotic translation initiation factor 6; eIF-6; B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP)
Entrez Gene (Human)：
3692;
Entrez Gene (Mouse)：
16418;
Entrez Gene (Rat)：
305506;
SwissProt (Human)：
P56537;
SwissProt (Mouse)：
O55135;
SwissProt (Rat)：
Q3KRD8;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EIF6 expression in Hela (A), HepG2 (B), K562 (C) whole cell lysates. (Predicted band size: 23; 26 kD; Observed band size: 27 kD) -
Immunofluorescent analysis of EIF6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.