Description:Rabbit polyclonal antibody to Cytochrome P450 2F1Immunogen:Recombinant full length protein of human Cytochrome P450 2F1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:CYP2F1Alternative Names:Cytochrome P450 2F1; CYPIIF1
Entrez Gene (Human):
1572;
SwissProt (Human):
P24903;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cytochrome P450 2F1 expression in SW620 (A), BT474 (B), mouse lung (C), mouse liver (D), rat brain (E) whole cell lysates. (Predicted band size: 36; 55 kD; Observed band size: 46-54 kD)
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Immunohistochemical analysis of Cytochrome P450 2F1 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.