Datasheet
MSDSDescription：Rabbit polyclonal antibody to ChitotriosidaseImmunogen：Recombinant full length protein of human ChitotriosidasePurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：CHIT1Alternative Names：Chitotriosidase-1; Chitinase-1
Entrez Gene (Human)：
1118;
SwissProt (Human)：
Q13231;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Chitotriosidase expression in U251 (A), HEK293T (B), mouse spleen (C) whole cell lysates. (Predicted band size: 43; 48; 49; 51 kD; Observed band size: 55 kD) -
Immunofluorescent analysis of Chitotriosidase staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).