Description:Rabbit polyclonal antibody to CCT3Immunogen:Recombinant full length protein of human CCT3Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:CCT3Alternative Names:CCTG; TRIC5; T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5
Entrez Gene (Human):
7203;
Entrez Gene (Mouse):
12462;
Entrez Gene (Rat):
295230;
SwissProt (Human):
P49368;
SwissProt (Mouse):
P80318;
SwissProt (Rat):
Q6P502;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CCT3 expression in MCF7 (A), THP1 (B), mouse ovary (C), mouse thymus (D), rat spleen (E), rat heart (F) whole cell lysates. (Predicted band size: 56; 60 kD; Observed band size: 60 kD)
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Immunofluorescent analysis of CCT3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.