Description：Rabbit polyclonal antibody to BARImmunogen：Recombinant full length protein of human BARPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：BFARAlternative Names：BAR; RNF47; Bifunctional apoptosis regulator; RING finger protein 47 Entrez Gene (Human)： 51283; Entrez Gene (Mouse)： 67118; Entrez Gene (Rat)： 304709; SwissProt (Human)： Q9NZS9; SwissProt (Mouse)： Q8R079; SwissProt (Rat)： Q5PQN2; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BAR expression in A549 (A), SHSY5Y (B), mouse brain (C), mouse liver (D), rat heart (E) whole cell lysates. (Predicted band size: 38; 52 kD; Observed band size: 53 kD)
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  Immunofluorescent analysis of BAR staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).