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CPA4044
  • Western blot analysis of JUNB (Phospho-S259) expression in mouse liver (A), mouse kidney (B), rat kidney (C) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 43 kD)
  • Immunohistochemical analysis of JUNB (Phospho-S259) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-JUNB (Phospho-S259) Antibody
Cat No:CPA4044
Source:Rabbit
Reactivity:H, M, R, B, S
Applications:WB, IH, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to JUNB (Phospho-S259)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S259 of human JUNB protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Gene Symbol:JUNB
Alternative Names:Transcription factor jun-B
Entrez Gene (Human): 3726;
Entrez Gene (Mouse): 16477;
Entrez Gene (Rat): 24517;
SwissProt (Human): P17275;
SwissProt (Mouse): P09450;
SwissProt (Rat): P24898;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of JUNB (Phospho-S259) expression in mouse liver (A), mouse kidney (B), rat kidney (C) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 43 kD)
  • Immunohistochemical analysis of JUNB (Phospho-S259) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
GSK3-SCF(FBXW7) targets JunB for degradation in G2 to preserve chromatid cohesion before anaphase
Journal Oncogene
IF 8
Application
Reactivity
PMID 22710716
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