Description:Rabbit polyclonal antibody to IRX3Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IRX3. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:IRX3Alternative Names:IRXB1; Iroquois-class homeodomain protein IRX-3; Homeodomain protein IRXB1; Iroquois homeobox protein 3
Entrez Gene (Human):
79191;
Entrez Gene (Mouse):
16373;
SwissProt (Human):
P78415;
SwissProt (Mouse):
P81067;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IRX3 expression in Hela (A), A549 (B) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)
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Immunohistochemical analysis of IRX3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.