Description：Rabbit polyclonal antibody to DUSP16Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DUSP16. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Gene Symbol：DUSP16Alternative Names：KIAA1700; MKP7; Dual specificity protein phosphatase 16; Mitogen-activated protein kinase phosphatase 7; MAP kinase phosphatase 7; MKP-7 Entrez Gene (Human)： 80824; SwissProt (Human)： Q9BY84; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DUSP16 expression in H1688 (A), mouse kidney (B), mouse brain (C), rat kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 73 kD)
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  Immunohistochemical analysis of DUSP16 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of DUSP16 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.