Description：Rabbit polyclonal antibody to ANAPC1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ANAPC1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：ANAPC1Alternative Names：TSG24; Anaphase-promoting complex subunit 1; APC1; Cyclosome subunit 1; Mitotic checkpoint regulator; Testis-specific gene 24 protein Entrez Gene (Human)： 64682; Entrez Gene (Mouse)： 17222; SwissProt (Human)： Q9H1A4; SwissProt (Mouse)： P53995; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ANAPC1 expression in HCT116 (A), PC12 (B), CT26 (C) whole cell lysates. (Predicted band size: 216 kD; Observed band size: 243 kD)
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  Immunofluorescent analysis of ANAPC1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.