Description：Rabbit polyclonal antibody to RAD50Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD50. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：RAD50Alternative Names：DNA repair protein RAD50; hRAD50 Entrez Gene (Human)： 10111; Entrez Gene (Rat)： 64012; SwissProt (Human)： Q92878; SwissProt (Mouse)： P70388; SwissProt (Rat)： Q9JIL8; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of RAD50 expression in HeLa (A), K562 (B), Jurkat (C), MCF7 (D), Ramos (E), SW480 (F) whole cell lysates. (Predicted band size: 138; 153; 154 kD; Observed band size: 154 kD)
• 
  Immunofluorescent analysis of RAD50 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.