Description：Rabbit polyclonal antibody to G3BP2Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human G3BP2. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：G3BP2Alternative Names：KIAA0660; Ras GTPase-activating protein-binding protein 2; G3BP-2; GAP SH3 domain-binding protein 2 Entrez Gene (Human)： 9908; Entrez Gene (Mouse)： 23881; SwissProt (Human)： Q9UN86; SwissProt (Mouse)： P97379; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of G3BP2 expression in MCF7 (A), SKOVCAR3 (B), A549 (C), C6 (D), CT26 (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54; 51 kD)
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  Immunofluorescent analysis of G3BP2 staining in VB2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).