Datasheet
MSDSDescription：Rabbit polyclonal antibody to Semaphorin 3EImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Semaphorin 3E. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：SEMA3EAlternative Names：KIAA0331; SEMAH; Semaphorin-3E
Entrez Gene (Human)：
9723;
Entrez Gene (Mouse)：
20349;
SwissProt (Human)：
O15041;
SwissProt (Mouse)：
P70275;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Semaphorin 3E expression in A549 (A), mouse liver (B), rat liver (C) whole cell lysates. (Predicted band size: 89 kD; Observed band size: 89 kD) -
Immunofluorescent analysis of Semaphorin 3E staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).