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CPA2415
  • Western blot analysis of N4BP1 expression in AML12 (A), PC12 (B), HEK293T (C), A549 (D), A2780 (E) whole cell lysates. (Predicted band size: 100 kD; Observed band size: 100 kD)
  • Immunohistochemical analysis of N4BP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-N4BP1 Antibody
Cat No:CPA2415
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to N4BP1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human N4BP1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Gene Symbol:N4BP1
Alternative Names:KIAA0615; NEDD4-binding protein 1; N4BP1
Entrez Gene (Human): 9683;
Entrez Gene (Mouse): 80750;
SwissProt (Human): O75113;
SwissProt (Mouse): Q6A037;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of N4BP1 expression in AML12 (A), PC12 (B), HEK293T (C), A549 (D), A2780 (E) whole cell lysates. (Predicted band size: 100 kD; Observed band size: 100 kD)
  • Immunohistochemical analysis of N4BP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Lys-63-linked Ubiquitination by E3 Ubiquitin Ligase Nedd4-1 Facilitates Endosomal Sequestration of Internalized a-Synuclein
Journal J Biol Chem
IF 4.8
Application WB, ICC/IF
Reactivity
PMID 24831002
A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV
Journal eLife
IF 7.616
Application WB
Reactivity
PMID 30520725
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