Description：Rabbit polyclonal antibody to ROCK2Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ROCK2. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100)Gene Symbol：ROCK2Alternative Names：KIAA0619; Rho-associated protein kinase 2; Rho kinase 2; Rho-associated coiled-coil-containing protein kinase 2; Rho-associated coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2 Entrez Gene (Human)： 9475; Entrez Gene (Mouse)： 19878; Entrez Gene (Rat)： 25537; SwissProt (Human)： O75116; SwissProt (Mouse)： P70336; SwissProt (Rat)： Q62868; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of ROCK2 expression in MCF7 (A), HeLa (B), NIH3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 160 kD; Observed band size: 161 kD)
• 
  Immunohistochemical analysis of ROCK2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• 
  Immunofluorescent analysis of ROCK2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).