Description:Rabbit polyclonal antibody to GEF H1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GEF H1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:ARHGEF2Alternative Names:KIAA0651; LFP40; Rho guanine nucleotide exchange factor 2; Guanine nucleotide exchange factor H1; GEF-H1; Microtubule-regulated Rho-GEF; Proliferating cell nucleolar antigen p40
Entrez Gene (Human):
9181;
Entrez Gene (Mouse):
16800;
Entrez Gene (Rat):
310635;
SwissProt (Human):
Q92974;
SwissProt (Mouse):
Q60875;
SwissProt (Rat):
Q5FVC2;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GEF H1 expression in SHSY5Y (A), mouse liver (B), mouse testis (C), rat spleen (D), rat testis (E) whole cell lysates. (Predicted band size: 111 kD; Observed band size: 111 kD)
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Immunohistochemical analysis of GEF H1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.