Description：Rabbit polyclonal antibody to ARHGEF1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARHGEF1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：ARHGEF1Alternative Names：Rho guanine nucleotide exchange factor 1; 115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5 Entrez Gene (Human)： 9138; Entrez Gene (Mouse)： 16801; SwissProt (Human)： Q92888; SwissProt (Mouse)： Q61210; SwissProt (Rat)： Q9Z1I6; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ARHGEF1 expression in HuT78 (A), A549 (B) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 125; 90 kD)
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  Immunohistochemical analysis of ARHGEF1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.