Description:Rabbit polyclonal antibody to PCAFImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PCAF. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:KAT2BAlternative Names:PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Histone acetylase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor; P/CAF
Entrez Gene (Human):
8850;
Entrez Gene (Mouse):
18519;
SwissProt (Human):
Q92831;
SwissProt (Mouse):
Q9JHD1;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PCAF expression in HEK293T (A), SGC7901 (B), Panc1 (C), PC12 (D), C6 (E) whole cell lysates. (Predicted band size: 93 kD; Observed band size: 93 kD)
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Immunohistochemical analysis of PCAF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.