Description:Rabbit polyclonal antibody to IKK gamma (Phospho-S85)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S85 of human IKK gamma protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:IKBKGAlternative Names:FIP3; NEMO; NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF-kappa-B essential modifier
Entrez Gene (Human):
8517;
Entrez Gene (Mouse):
16151;
Entrez Gene (Rat):
309295;
SwissProt (Human):
Q9Y6K9;
SwissProt (Mouse):
O88522;
SwissProt (Rat):
Q6TMG5;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IKK gamma (Phospho-S85) expression in THP1 LPS-treated (A), Raw264.7 LPS-treated (B), rat brain (C) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 48 kD)
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Immunohistochemical analysis of IKK gamma (Phospho-S85) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.