Description:Rabbit polyclonal antibody to SLC43A1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SLC43A1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol:SLC43A1Alternative Names:LAT3; PB39; POV1; Large neutral amino acids transporter small subunit 3; L-type amino acid transporter 3; Prostate cancer overexpressed gene 1 protein; Solute carrier family 43 member 1
Entrez Gene (Human):
8501;
Entrez Gene (Mouse):
72401;
SwissProt (Human):
O75387;
SwissProt (Mouse):
Q8BSM7;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of SLC43A1 expression in HEK293T (A), SP2/0 (B), H9C2 (C) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 61 kD)
-
Immunofluorescent analysis of SLC43A1 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).