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CPA2223
  • Western blot analysis of Ezrin (Phospho-T567) expression in HeLa (A), A431 (B), SHSY5Y (C) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 80 kD)
  • Immunohistochemical analysis of Ezrin (Phospho-T567) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Ezrin (Phospho-T567) Antibody
Cat No:CPA2223
Source:Rabbit
Reactivity:H, M, R, B, C, P, Z
Applications:WB, IH, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to Ezrin (Phospho-T567)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T567 of human Ezrin protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Gene Symbol:EZR
Alternative Names:VIL2; Ezrin; Cytovillin; Villin-2; p81
Entrez Gene (Human): 7430;
Entrez Gene (Mouse): 22350;
Entrez Gene (Rat): 54319;
SwissProt (Human): P15311;
SwissProt (Mouse): P26040;
SwissProt (Rat): P31977;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Ezrin (Phospho-T567) expression in HeLa (A), A431 (B), SHSY5Y (C) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 80 kD)
  • Immunohistochemical analysis of Ezrin (Phospho-T567) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Journal J Am Soc Nephrol
IF 13.6
Application
Reactivity
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Akt/Ezrin Tyr353/NF-?B pathway regulates EGF-induced EMT and metastasis in tongue squamous cell carcinoma
Journal Br J Cancer
IF 8.8
Application
Reactivity
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Increased phosphorylation of ezrin is associated with the migration and invasion of fibroblast-like synoviocytes from patients with rheumatoid arthritis
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