Description:Rabbit polyclonal antibody to p73 (Phospho-Y99)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y99 of human p73 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:TP73Alternative Names:P73; Tumor protein p73; p53-like transcription factor; p53-related protein
Entrez Gene (Human):
7161;
SwissProt (Human):
O15350;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p73 (Phospho-Y99) expression in Jurkat (A), HeLa UV-treated (B) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 80 kD)
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Immunohistochemical analysis of p73 (Phospho-Y99) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.