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CPA2170
  • Western blot analysis of TLE4 expression in HeLa (A), Jurkat (B), HEK293T (C) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 84 kD)
  • Immunohistochemical analysis of TLE4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-TLE4 Antibody
Cat No:CPA2170
Source:Rabbit
Reactivity:H, M, R, C
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to TLE4
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:TLE4
Alternative Names:KIAA1261; Transducin-like enhancer protein 4
Entrez Gene (Human): 7091;
Entrez Gene (Mouse): 21888;
Entrez Gene (Rat): 25565;
SwissProt (Human): Q04727;
SwissProt (Mouse): Q62441;
SwissProt (Rat): Q07141;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of TLE4 expression in HeLa (A), Jurkat (B), HEK293T (C) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 84 kD)
  • Immunohistochemical analysis of TLE4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Tle4 regulates epigenetic silencing of gamma interferon expression during effector T helper cell tolerance
Journal Mol Cell Biol
IF 5.3
Application
Reactivity
PMID 24190972
The corepressor tle4 is a novel regulator of murine hematopoiesis and bone development
Journal PLoS One
IF 3.7
Application WB
Reactivity Mouse
PMID 25153823
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