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CPA2032
  • Western blot analysis of S6K1 expression in MCF7 (A), HeLa (B), MDCK (C), mouse brain (D) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 70 kD)
  • Immunohistochemical analysis of S6K1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-S6K1 Antibody
Cat No:CPA2032
Source:Rabbit
Reactivity:H, M, R, B, Rb
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to S6K1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human S6K1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:RPS6KB1
Alternative Names:STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
Entrez Gene (Human): 6198;
Entrez Gene (Mouse): 72508;
Entrez Gene (Rat): 83840;
SwissProt (Human): P23443;
SwissProt (Mouse): Q8BSK8;
SwissProt (Rat): P67999;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of S6K1 expression in MCF7 (A), HeLa (B), MDCK (C), mouse brain (D) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 70 kD)
  • Immunohistochemical analysis of S6K1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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