Welcome to Cohesion Biosciences
Home> Products > Primary Antibody
CPA1998
  • Western blot analysis of NF-kappaB p65 (Phospho-S468) expression in HeLa TNFa-treated (A), NIH3T3 LPS-treated (B), rat brain (C) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 65 kD)
  • Immunohistochemical analysis of NF-kappaB p65 (Phospho-S468) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-NF-kappaB p65 (Phospho-S468) Antibody
Cat No:CPA1998
Source:Rabbit
Reactivity:H, M, R, B, Mk, P
Applications:WB, IH, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
Add to cart Ship in 3 days My orders
Description:Rabbit polyclonal antibody to NF-kappaB p65 (Phospho-S468)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S468 of human NF-kappaB p65 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Gene Symbol:RELA
Alternative Names:NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Entrez Gene (Human): 5970;
Entrez Gene (Mouse): 19697;
SwissProt (Human): Q04206;
SwissProt (Mouse): Q04207;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of NF-kappaB p65 (Phospho-S468) expression in HeLa TNFa-treated (A), NIH3T3 LPS-treated (B), rat brain (C) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 65 kD)
  • Immunohistochemical analysis of NF-kappaB p65 (Phospho-S468) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Glucose promotes the production of interleukine-1beta and cyclooxygenase-2 in mesangial cells via enhanced (Pro)renin receptor expression
Journal Endocrinology
IF 4.8
Application WB
Reactivity Rat
PMID 19861503
PPM1A is a RelA phosphatase with tumor suppressor-like activity
Journal Oncogene
IF 8
Application
Reactivity
PMID 23812431
COHESION BIOSCIENCES LIMITED
Copyright © 2022 Cohesion Biosciences. All Rights Reserved