Description：Rabbit polyclonal antibody to c-RAFImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-RAF. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：RAF1Alternative Names：RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1 Entrez Gene (Human)： 5894; Entrez Gene (Mouse)： 110157; Entrez Gene (Rat)： 24703; SwissProt (Human)： P04049; SwissProt (Mouse)： Q99N57; SwissProt (Rat)： P11345; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of c-RAF expression in A431 (A), HeLa (B), RAW264.7 (C), C6 (D) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 74 kD)
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  Immunofluorescent analysis of c-RAF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.