Description:Rabbit polyclonal antibody to Focal Adhesion KinaseImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Focal Adhesion Kinase. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:PTK2Alternative Names:FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
Entrez Gene (Human):
5747;
Entrez Gene (Mouse):
14083;
Entrez Gene (Rat):
25614;
SwissProt (Human):
Q05397;
SwissProt (Mouse):
P34152;
SwissProt (Rat):
O35346;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Focal Adhesion Kinase expression in SGC7901 (A), Panc1 (B), MCF7 (C), H1792 (D), A549 (E), 3T3L1 (F), H9C2 (G) whole cell lysates. (Predicted band size: 119; 99; 63; 48; 120; 39; 114 kD; Observed band size: 125 kD)
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Immunohistochemical analysis of Focal Adhesion Kinase staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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