Description：Rabbit polyclonal antibody to Focal Adhesion Kinase (Phospho-Y925)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y925 of human Focal Adhesion Kinase protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：PTK2Alternative Names：FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK Entrez Gene (Human)： 5747; Entrez Gene (Mouse)： 14083; Entrez Gene (Rat)： 25614; SwissProt (Human)： Q05397; SwissProt (Mouse)： P34152; SwissProt (Rat)： O35346; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Focal Adhesion Kinase (Phospho-Y925) expression in NIH3T3 pervanadate-treated (A) whole cell lysates. (Predicted band size: 119; 99; 63; 48; 120; 39; 114 kD; Observed band size: 125 kD)
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  Immunofluorescent analysis of Focal Adhesion Kinase (Phospho-Y925) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.